Morphometric analyses & DNA Barcoding of Acrididae (Orthoptera: Caelifera) using Cytochrome Oxidase Subunit I Gene

Abdul Aziz Babar; Riffat  Sultana; Santosh Kumar

doi:10.38211/joarps.2023.04.01.53

Authors

Abdul Aziz Babar Department of Zoology, University of Sindh, Jamshoro
Riffat Sultana Department of Zoology, Sindh University, JamShoro https://orcid.org/0000-0003-4775-8452
Santosh Kumar Department of Zoology, Cholistan University of Veterinary and Animal Sciences, Bahawalpur, Punjab

DOI:

https://doi.org/10.38211/joarps.2023.04.01.53

Keywords:

DNA bar-coding, biodiversity, Sequence, , Acrididae, Sindh

Abstract

DNA barcode data of Acrididae is limited in global data bases from Sindh, Pakistan. Hence, the present study was aimed to collect some selected Acridid species from the Sindh and generate DNA barcode data to improve the global database sequencing. Specimens were collected from cultivated, sandy, rocky, vegetation, grassy, desert, semi desert, roadside and open area of the upper Sindh districts i.e., Dadu, Ghotki, Jacobabad, Kashmore, Khairpur Miras, Larkana, Naushahro Feroz, Qambar & Shahdadkot, Shikarpur, Sukkur. Seven species of Acrididae were under discussion i.e., (24%) followed by Acrida willemsei (19%), Truxalis eximia eximia (15%), Aiolopus thalassinus thalassinus (13%), Acrotylus longipes longipes (10%), Aiolopussimulatrix (10%) and Sphingonotus savignyi (09%) belong to 3 sub-families i.e., Acridinae, Oedipodinae and Oxyinae. Moreover, maximum number of specimens belonged to Oxyinae. The present study suggests the collection of multiple specimens from different geographical locations and the generation of more DNA barcode data would facilitate the actual diversity amongst this taxon.

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