DNA Barcoding Based Identification of Rosa x Damascene and Prunus dulcis Herbs Using ITS2 Barcoding Gene Amplification.

Authors

  • Syed Rizwan Department of Human Nutrition & dietetics, Faculty of Eastern Medicine, Hamdard University
  • Hakim Abdul Bari Hamdard Laboratories (Waqf) Pakistan, Karachi-75270, Pakistan.
  • Arshad Saleem Hamdard Laboratories (Waqf) Pakistan, Karachi-75270, Pakistan
  • Song Jingyuan Key Lab of Chinese Medicine Resources Conservation, State Administration of Traditional Chinese Medicine of the People’s Republic of China, Engineering Research Center of Chinese Medicine Resources of Ministry of Education, Institute of Medicinal Plant Development, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100193, China
  • Xin Tianyi Key Lab of Chinese Medicine Resources Conservation, State Administration of Traditional Chinese Medicine of the People’s Republic of China, Engineering Research Center of Chinese Medicine Resources of Ministry of Education, Institute of Medicinal Plant Development, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100193, China
  • Muhammad Jahanzeb Hamdard Laboratories (Waqf) Pakistan, Karachi-75270, Pakistan.

DOI:

https://doi.org/10.38211/joarps.2024.05.219

Abstract

The considerable risk of adulteration in the herbs has raised commercial interest in the identification of medicinal herbs globally. DNA barcoding is the primary techniques for identifying the herbs at genetic level. This technique's key benefit is that it can identify the material's purity. This study focuses on the accurate identification of species utilizing Polymerase chain reaction-based nuclear universal internal transcribed spacer region (a barcode region) amplification and sequencing in 2 medically significant plants (Rosa damascene and Prunus dulcis) procured from the local herbal market of Karachi in the year 2022. Results suggest that the ribosomal nuclear ITS2 region of the selected plant species shows the 100 % identity with the reference genome, therefore it has shown a good rate of identification at the species level. The findings of this concludes that ITS2 is the novel standard barcode that involve in species identification, genomic conservation, and secure utilization of the medically significant plant species.

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Published

2024-01-06

How to Cite

Rizwan, S., Bari, H. A., Saleem, A., Jingyuan, S., Tianyi, X., & Jahanzeb, M. (2024). DNA Barcoding Based Identification of Rosa x Damascene and Prunus dulcis Herbs Using ITS2 Barcoding Gene Amplification. Journal of Applied Research in Plant Sciences , 5(01), 58–62. https://doi.org/10.38211/joarps.2024.05.219