Evaluation of soma-clonal variation in CP-671026 mutant of sugarcane (Saccharum officinrum L) through morphological techniques

Zohra Fatima Memon; Shala Baloch; Muharam Ali; Imtiyaz Ahmed Khan; Allah Jurio  Khaskheli; Shafquat Yasmine; Sadaruddin Chachar; Zareen Qambrani; Bilqees Magsi; Sayyed Zakir Hussain Shah

doi:10.38211/joarps.2023.04.01.59

Authors

Zohra Fatima Memon Department of Biotechnology, Faculty of Crop Production, Sindh Agriculture University, TandoJam-70060, Pakistan
Shala Baloch Department of Biotechnology, SAU, Tandojam
Muharam Ali Department of Biotechnology, Faculty of Crop Production, Sindh Agriculture University, TandoJam-70060, Pakistan
Imtiyaz Ahmed Khan Nuclear Institute of Agriculture NIA, Tando Jam, Hyderabad, Sindh, Pakistan
Allah Jurio Khaskheli Department of Biotechnology, Faculty of Crop Production, Sindh Agriculture University, TandoJam-70060, Pakistan
Shafquat Yasmine Nuclear Institute of Agriculture NIA, Tando Jam, Hyderabad, Sindh, Pakistan
Sadaruddin Chachar Department of Biotechnology, Faculty of Crop Production, Sindh Agriculture University, TandoJam-70060, Pakistan
Zareen Qambrani Department of Biotechnology, Faculty of Crop Production, Sindh Agriculture University, TandoJam-70060, Pakistan
Bilqees Magsi Department of Biotechnology, Faculty of Crop Production, Sindh Agriculture University, TandoJam-70060, Pakistan
Sayyed Zakir Hussain Shah Department of Biotechnology, Faculty of Crop Production, Sindh Agriculture University, TandoJam-70060, Pakistan

DOI:

https://doi.org/10.38211/joarps.2023.04.01.59

Keywords:

CP-671026 mutant, Morphological techniques, Soma-clonal variation, Sugarcane

Abstract

Present studies were conducted on evaluation of soma-clonal variation of CP67-1026 mutant through morphological procedures with the aims at discussing how soma-clonal variations can be analyzed through morphological approaches in gaining knowledge about the underlined regulatory controlled mechanisms in plants. In vitro experiments were conducted in the Laboratory of Nuclear Institute Atomic Energy (NIA), Tandojam during the year of 2021. The results so far achieved opened further window for deep analysis with reference to functional analysis of soma-clonal variated plants with different approaches. Results on the performance for soma-clonal variation in sugarcane variety NIA-2010 and their soma-clones revealed the best performance of varieties, which showed that the plant height (302.33cm) was observed in SC-3 and the lowest performance was examined in SC-2 that was approximately (154.33cm). The consequences of varieties and diverse parameters indicated that the highest number of internodes plant^-1 were recorded (38) in SC-3 and lowest number of internodes plant^-1 were accomplished (23) in SC-1. The output of different features indicated that highest number of tillers plant^-1 was observed (8.02) in SC-3, the lowest number of tillers plant^-1 (4.33) were noted in SC-4 and 8 as well. The consequences of soma-clones indicated that maximum weight stool^-1 (kg) was observed (9.67kg) in SC-3 and minimum weight stool^-1 (kg) was showed (5.83) in SC-1. The output of different physiological features indicated that highest values were remarkably noticed of brix (%) (19.83%) in SC-13, sucrose (%) (13.4%) in SC-3, fiber (%) (18.92g) in SC-3 whereas the lowest fiber (%) (16.58g) were seen in SC-11. The results of various agronomic parameters such as germination % observed (82.01%) in SC-6, commercial cane sugar percentage (CCS %) showed (9.1%) in SC-9, sugar recovery (%) (8.56%) in SC-8, purity (%) (85.27%) in SC-8, cane yield (tonnes per hectare) (96.67 tonnes per hectare) in SC-3, sugar yield (tonnes per hectare) (7.95 tonnes per hectare) in SC-3 for the assessment of morphological constitutions of NIA-2010 genotype and their soma-clone

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