Iron Biofortification of Wheat Grains with Various Iron Fertilization Techniques

Authors

  • Saima Kalsoom Babar Department of Soil Science Sindh Agriculture University Tandojam Sindh https://orcid.org/0000-0002-5600-6503 (unauthenticated)
  • Zeeshan Mashori Department of Soil Science, Sindh Agriculture University Tandojam, 70060, Pakistan
  • Nizamuddin Depar Soil & Environmental Sciences Division, Nuclear Institute of Agriculture, Tandojam, Pakistan
  • Aydin Gunes Department of Soil Science & Plant Nutrition, Ankara University, 06110 Turkiye
  • Hassan Ali Shujrah Department of Soil Science, Sindh Agriculture University Tandojam, 70060, Pakistan

DOI:

https://doi.org/10.38211/joarps.2025.06.318

Keywords:

malnutrition, biofortification, Fe-application, novel variety, calcareous soil

Abstract

Most Pakistani soils are deficient in available iron (Fe), which limits crop productivity. Biofortification offers a sustainable solution by enhancing nutrient content in edible parts. This field experiment, conducted at the Nuclear Institute of Agriculture (NIA), Tandojam during the 2023–24 Rabi season, evaluated the effectiveness of iron application methods on wheat (variety NBW-232). The study used a randomized complete block design (RCBD) with four treatments and three replications on silty loam soil. Treatments included: T1 (control), T2 (12 kg Fe ha⁻¹ via broadcasting), T3 (12 kg Fe ha⁻¹ as Fe-coated urea), and T4 (seed priming with 2% FeSO₄·7H₂O). A uniform NPK dose (150-80-50 kg ha⁻¹) was applied. Results showed that Fe application significantly improved growth, yield, and Fe accumulation, with Fe-coated urea (T3) being the most effective. Iron coated urea (T3) succeeded to obtain the highest number of grains per spike (60.2), grain yield (5533.3 kg ha⁻¹), nitrogen (2.9%), and Fe content (119.3 mg kg⁻¹), while reducing phytic acid (1.807 mg g⁻¹) concentration (p<0.01). In conclusion, Fe-coated urea markedly enhanced wheat productivity and its nutritional value

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References

Babar, S. K., Abbasi, Z., Rajper, I., Babar, H., & Shujrah, H. A. (2025). Efficiency of Nano-silicon Coated Urea with Various Fertilizing Techniques on Maize (Zea mays L.) Performance and Nitrogen Content. Journal of Applied Research in Plant Sciences, 6(1), 01-07. DOI: https://doi.org/10.38211/joarps.2025.06.288

Babar, S. K., Akca, H., Taban, S., & Shujrah, H. A. (2024). Effect of Zn Applied with or Without Palm Stearin Coated Urea on The Growth and Mineral Element Concentration of Maize (Zea mays L.). Journal of Applied Research in Plant Sciences, 5(01), 119-125. DOI: https://doi.org/10.38211/joarps.2024.05.252

Baral, A., Pesce, C., Zhakiyeva, Z., Snellings, R., Hanein, T., Provis, J. L., & Peys, A. (2024). Characterisation of iron-rich cementitious materials. Cement and Concrete Research, 177, p.107419. DOI: https://doi.org/10.1016/j.cemconres.2023.107419

Bhatti, S. M. B., Depar, N., Rajpar, I., & Sarki, M. S. (2021). Enhancement of zinc concentration and bioavailability in rice grains by zinc application methods. Pakistan Journal of Agricultural Sciences, 58(5). DOI: https://doi.org/10.21162/PAKJAS/21.9922

Bouis, H. E., Hotz, C., McClafferty, B., Meenakshi, J. V., & Pfeiffer, W. H. (2011). Biofortification: a new tool to reduce micronutrient malnutrition. Food and nutrition bulletin, 32(1_suppl1), S31-S40. DOI: https://doi.org/10.1177/15648265110321S105

Bouis, H. E., & Welch, R. M. (2010). Biofortification—a sustainable agricultural strategy for reducing micronutrient malnutrition in the global south. Crop science, 50, S-20. DOI: https://doi.org/10.2135/cropsci2009.09.0531

Chakraborty, B., Singh, P. N., Kumar, S., & Srivastava, P. C. (2014). Uptake and distribution of iron from different iron sources applied as foliar sprays to chlorotic leaves of low-chill peach cultivars. Agricultural Research, 3(4), 293-301. DOI: https://doi.org/10.1007/s40003-014-0128-4

Ciccolini, V., Pellegrino, E., Coccina, A., Fiaschi, A. I., Cerretani, D., Sgherri, C., & Ercoli, L. (2017). Biofortification with iron and zinc improves nutritional and nutraceutical properties of common wheat flour and bread. Journal of agricultural and food chemistry, 65(27), 5443-5452. DOI: https://doi.org/10.1021/acs.jafc.7b01176

Eagling, T., Wawer, A. A., Shewry, P. R., Zhao, F. J., & Fairweather-Tait, S. J. (2014). Iron bioavailability in two commercial cultivars of wheat: comparison between wholegrain and white flour and the effects of nicotianamine and 2′-deoxymugineic acid on iron uptake into Caco-2 cells. Journal of agricultural and food chemistry, 62(42), 10320-10325. DOI: https://doi.org/10.1021/jf5026295

Gupta, P. K., Balyan, H. S., Sharma, S., & Kumar, R. (2021). Biofortification and bioavailability of Zn, Fe and Se in wheat: present status and future prospects. Theoretical and Applied Genetics, 134(1), 1-35. DOI: https://doi.org/10.1007/s00122-020-03709-7

Haug, W., & Lantzsch, H. J. (1983). Sensitive method for the rapid determination of phytate in cereals and cereal products. Journal of the Science of Food and Agriculture, 34(12), 1423-1426. DOI: https://doi.org/10.1002/jsfa.2740341217

Houssni, I. E., Zahidi, A., Khedid, K., & Hassikou, R. (2024). Review of processes for improving the bioaccessibility of minerals by reducing the harmful effect of phytic acid in wheat. Food Chemistry Advances, 4, 100568. DOI: https://doi.org/10.1016/j.focha.2023.100568

Kiran, A., Wakeel, A., Mahmood, K., Mubaraka, R., Hafsa, & Haefele, S. M. (2022). Biofortification of staple crops to alleviate human malnutrition: contributions and potential in developing countries. Agronomy, 12(2), 452. DOI: https://doi.org/10.3390/agronomy12020452

Krishnan, R., & Meera, M. S. (2017). Assessment of inhibitory factors on bioaccessibility of iron and zinc in pearl millet (Pennisetum glaucum (L.) R. Br.) cultivars. Journal of food science and technology, 54(13), 4378-4386. DOI: https://doi.org/10.1007/s13197-017-2911-2

Kumar, M., Kumar, D., Sharma, A., Bhadauria, S., Thakur, A., & Bhatia, A. (2024). Micronutrients throughout the life cycle: needs and functions in health and disease. Current Nutrition & Food Science, 20(1), 62-84. DOI: https://doi.org/10.2174/1573401319666230420094603

Kutman, U. B., Yildiz, B., & Cakmak, I. (2011). Effect of nitrogen on uptake, remobilization and partitioning of zinc and iron throughout the development of durum wheat. Plant and Soil, 342(1), 149-164. DOI: https://doi.org/10.1007/s11104-010-0679-5

Magallanes-López, A. M., Hernandez-Espinosa, N., Velu, G., Posadas-Romano, G., Ordoñez-Villegas, V. M. G., Crossa, J., & Guzmán, C. (2017). Variability in iron, zinc and phytic acid content in a worldwide collection of commercial durum wheat cultivars and the effect of reduced irrigation on these traits. Food chemistry, 237, 499-505. DOI: https://doi.org/10.1016/j.foodchem.2017.05.110

Mahender, A., Swamy, B. M., Anandan, A., & Ali, J. (2019). Tolerance of iron-deficient and-toxic soil conditions in rice. Plants, 8(2), 31. DOI: https://doi.org/10.3390/plants8020031

Marschner, Horst. Marschner's mineral nutrition of higher plants. Academic press, 2012.

Mushtaq, Z., Alasmari, A., Demir, C., Oral, M. A., Bellitürk, K., & Baran, M. F. (2025). Enhancing iron content in potatoes: a critical strategy for combating nutritional deficiencies. Potato Research, 68(1), 715-741. DOI: https://doi.org/10.1007/s11540-024-09758-5

Naresh, R. K., Chandra, M. S., Mahajan, N. C., Singh, P. K., Baliyan, A., Ahlawat, P., & Shalini, P. (2021). Neglected and underutilized crop species the key to improving soil nutritional security for fighting poverty, hunger and malnutrition in north-western IGP: a review. Pharma Innovation Journal, 10(8), 1833-1839.

Naveed, M., Khalid, H., Ayub, M. A., Rehman, M. Z. U., Rizwan, M., Rasul, A., & Haq, M. A. U. (2020). Biofortification of cereals with zinc and iron: recent advances and future perspectives. Resources use efficiency in agriculture, 615-646. DOI: https://doi.org/10.1007/978-981-15-6953-1_17

Pal, V., Singh, G., & Dhaliwal, S. S. (2021). A new approach in agronomic biofortification for improving zinc and iron content in chickpea (Cicer arietinum L.) grain with simultaneous foliar application of zinc sulphate, ferrous sulphate and urea. Journal of Soil Science and Plant Nutrition, 21(2), 883-896. DOI: https://doi.org/10.1007/s42729-021-00408-0

Ramzan, Y., Hafeez, M. B., Khan, S., Nadeem, M., Batool, S., & Ahmad, J. (2020). Biofortification with zinc and iron improves the grain quality and yield of wheat crop. International Journal of Plant Production, 14(3), 501-510. DOI: https://doi.org/10.1007/s42106-020-00100-w

Riaz, A., Huda, N., Abbas, A., & Raza, S. (2017). Biofortification of wheat with iron. International Journal Advance Science Research, 3, 69-76. DOI: https://doi.org/10.7439/ijasr.v3i7.4275

Rios, J. J., Carrasco-Gil, S., Abadía, A., & Abadía, J. (2016). Using Perls staining to trace the iron uptake pathway in leaves of a Prunus rootstock treated with iron foliar fertilizers. Frontiers in Plant Science, 7, 893. DOI: https://doi.org/10.3389/fpls.2016.00893

Ryan, J., Estefan, G., & Rashid, A. (2007). Soil and plant analysis laboratory manual. ICARDA Aleppo

Sharma, V., Kumar, R. R., Pandey, R., & Singh, B. (2018). Regulation of phytosiderophore (PS) and Yellow Stripe-1 (YS1) transporter activity by sulphur (S) and that of high-affinity sulphate (SULTR1; 1) transporter by iron (Fe) in wheat. Internation Journal of Current Microbiology & Applied Sciences, 7(1), 71-88. DOI: https://doi.org/10.20546/ijcmas.2018.701.010

Singh, B. K., Delgado-Baquerizo, M., Egidi, E., Guirado, E., Leach, J. E., Liu, H., & Trivedi, P. (2023). Climate change impacts on plant pathogens, food security and paths forward. Nature Reviews Microbiology, 21(10), 640-656. DOI: https://doi.org/10.1038/s41579-023-00900-7

Statistix. 2006. Statistix 8 user guide, version 1.0. Analytical Software, PO Box 12185, Tallahassee FL 32317 USA. Copyright © 2006 by Analytical Software.

Szerement, J., Szatanik-Kloc, A., Mokrzycki, J., & Mierzwa-Hersztek, M. (2022). Agronomic biofortification with Se, Zn, and Fe: An effective strategy to enhance crop nutritional quality and stress defense A review. Journal of Soil Science and Plant Nutrition, 22(1), 1129-1159. DOI: https://doi.org/10.1007/s42729-021-00719-2

Taskin, Mehmet Burak, and Aydin Gunes (2022). Iron biofortification of wheat grains by foliar application of nano zero-valent iron (nZVI) and other iron sources with urea. Journal of Soil Science and Plant Nutrition, 22(4): 4642-4652. DOI: https://doi.org/10.1007/s42729-022-00946-1

Vashishth, A., Ram, S., & Beniwal, V. (2017). Cereal phytases and their importance in improvement of micronutrients bioavailability. 3 Biotech, 7(1), 42. DOI: https://doi.org/10.1007/s13205-017-0698-5

Wang, B., Yin, X., Wang, P., & Shen, L. (2023). Chemical looping ammonia synthesis at atmospheric pressure benefiting from synergistic effect of Mn-and Fe-based nitrogen carriers. International Journal of Hydrogen Energy, 48(7), 2705-2717. DOI: https://doi.org/10.1016/j.ijhydene.2022.10.132

Yang, J., & Zhang, J. (2006). Grain filling of cereals under soil drying. New phytologist, 169(2), 223-236. DOI: https://doi.org/10.1111/j.1469-8137.2005.01597.x

Zhao N, Zhang Q, Cheng F, Zhou W. (2007). Effects of nitrogen, phosphorus and zinc supply levels on grain phytic acid content and its correlation with several mineral nutrients in rice grains. Chinese Journal of Rice Science, 21(2):185–190.

Zimbovskaya, M. M., Polyakov, A. Y., Volkov, D. S., Kulikova, N. A., Lebedev, V. A., Pankratov, D. A., & Perminova, I. V. (2020). Foliar application of humic-stabilized nanoferrihydrite resulted in an increase in the content of iron in wheat leaves. Agronomy, 10(12), 1891. DOI: https://doi.org/10.3390/agronomy10121891

Zulfiqar, U., Maqsood, M., Hussain, S., & Anwar-ul-Haq, M. (2020). Iron nutrition improves productivity, profitability, and biofortification of bread wheat under conventional and conservation tillage systems. Journal of soil science and plant nutrition, 20(3), 1298-1310. DOI: https://doi.org/10.1007/s42729-020-00213-1

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Published

17-09-2025

How to Cite

Iron Biofortification of Wheat Grains with Various Iron Fertilization Techniques (S. K. Babar, Z. Mashori, N. Depar, A. Gunes, & H. A. Shujrah, Trans.). (2025). Journal of Applied Research in Plant Sciences, 6(02), 98-104. https://doi.org/10.38211/joarps.2025.06.318

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