Effects of Different Hormonal Concentrations on In vitro Regeneration and Multiplication of Pearl Millet (Pennisetum glaucum L.)

Rizwan Taj Khan; Robina Ilyas; Naveed Kamal; Saqib Saleem; Shahnaz Bibi; Muhammad Riaz Khan; Muhammad Tahir Younas; Matee ur Rehman Hafiz; Sami  Ullah; Basharat Mahmood

doi:10.38211/joarps.2022.3.2.38

Authors

Rizwan Taj Khan Department of Botany, University of Azad Jammu and Kashmir, Muzaffarabad, AJK, Pakistan
Robina Ilyas Department of Botany, University of Azad Jammu and Kashmir, Muzaffarabad, AJK, Pakistan
Naveed Kamal Wheat Research Sub-Station Murree
Saqib Saleem Potato Breeding Sub-Station, Murree, Pakistan
Shahnaz Bibi Department of Pure and Applied Biology, Bahahuddin Zakariya University Multan, Pakistan
Muhammad Riaz Khan Department of Horticulture, University of Poonch Rawalakot, AJK, Pakistan
Muhammad Tahir Younas Department of Plant Pathology
Matee ur Rehman Hafiz Department of Plant Pathology, University of Poonch Rawalakot, AJK, Pakistan
Sami Ullah Department of Plant Pathology, University of Poonch Rawalakot, AJK, Pakistan
Basharat Mahmood Department of Plant Pathology, University of Poonch Rawalakot, AJK, Pakistan

DOI:

https://doi.org/10.38211/joarps.2022.3.2.38

Abstract

An efficient in-vitro regeneration and multiplication protocol was developed to check the effects of different hormonal concentrations through somatic embryogenesis of Pearl millet (Pennisetum glaucum L.). The explants (seeds) of P. glaucum were surface sterilized with different concentrations (30, 50 and 70%) of Sodium hypochlorite to ensure the removal of surface contamination. For the regeneration the explants were inoculated on MS media supplemented with varying concentrations of indole acetic acid (IAA 2 mg/L, 0.75 mg/L, 0.1 mg/L, 0.2 mg/L and 0.3 mg/L) and kinetin (KIN 1.5 mg/L, 0.5 mg/L, 0.5 mg/L, 1 mg/L and 1.5mg/L) respectively. The maximum germination (87.5 %) of explants was obtained by using 70 % Chlorox and minimum germination (62.5 %) of explants was obtained by using 30% Chlorox. The maximum stem length (15.5 cm), roots number (12), roots length (3.96 cm) and leaves length (6.76 cm) was observed on MS medium containing (IAA 2 mg/L and KIN 1.5 mg/L). The maximum leaves number (7) was observed on MS medium containing (IAA 0.75 mg/L and KIN 0.5 mg/L). The minimum stem length (3 cm), roots number (1), roots length (1 cm), leaves number (1) and leaves length (3 cm) was observed on MS medium supplemented with (IAA 0.2 mg/L and KIN 1 mg/L). The maximum Stem length (15.5cm), Roots number (12), Roots length (3.96 cm) and Leaves length (6.76 cm) was observed on MS medium supplemented with (IAA 2 mg/L and KIN 1.5 mg/L) although maximum leaves number was observed on MS medium supplemented with (IAA 0.75 mg/L and KIN 0.5 mg/L). The study revealed an easy and reproducible in-vitro regeneration protocol of pearl millet that can provide an efficient plant regeneration which can be further exploited for transgenic applications

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Author Biography

Naveed Kamal, Wheat Research Sub-Station Murree

Researcher

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