Effect of Pretreatment, Fermentation Medium and Solid Loading Rate on The Production of Bio- Ethanol from Fruit Waste Using Saccharomyces cerevisiae

Muhammad Shahzaib; Muhammad Usman; Muhammad Moaaz Ali; Shaista Gull; Talha Javed; Ahmed Fathy Ahmed Yousef; Muhammad Waleed Shafique; Ayesha  Tahir

doi:10.38211/joarps.2021.2.2.16

Authors

Muhammad Shahzaib Department of Energy Systems Engineering, University of Agriculture Faisalabad 38000, Punjab, Pakistan
Muhammad Usman Department of Soil and Environmental Science, University of Agriculture Faisalabad 38000, Punjab, Pakistan
Muhammad Moaaz Ali College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China https://orcid.org/0000-0002-3092-6328
Shaista Gull Department of Horticulture, Bahauddin Zakariya University, Multan 66000, Punjab, Pakistan
Talha Javed College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China
Ahmed Fathy Ahmed Yousef College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China
Muhammad Waleed Shafique Department of Agronomy, University of New England, Armidale 2351, New South Wales, Australia
Ayesha Tahir Seed Physiology Lab, Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad 38000, Punjab, Pakistan

DOI:

https://doi.org/10.38211/joarps.2021.2.2.16

Keywords:

Fruit waste, Biofuel, Bio-ethanol, Renewable energy, Bioenergy, Simultaneous saccharification and fermentation (SSF)

Abstract

Rapid increase in world’s population and growing industrialization are major sources of energy consumption, therefore energy demand is expanding continuously. The first-generation feedstock like maize, sugarcane, wheat etc. can be used to produce bioethanol, but due to food and feed security issues first generation feedstock cannot be used to produce bioethanol. To overcome the feed and food security issue related to first-generation feedstock, waste fruit can be used to produce bioethanol. In this experiment, firstly the effect of pretreatment technique on glucose generation is observed. Simultaneous saccharification and fermentation (SSF) experiment carried out at a pH of 4.5 and temperature of 30^°C for 48 h with fermentation helping nutrients using Saccharomyces cerevisiae. Nearly equal amount of glucose concentration is observed from sample treated with hot water, 1% H₂SO₄, 5% H₂SO₄ and without any pretreatment. SSF results also revealed that fermentation helping nutrients has no significant effect on the production of bioethanol at same concentration. Second part of the experiment deals with the effect of solid loading rate, that is directly proportional to glucose concentration 10-20% (w/w) and time for fermentation (48-96 hours) on generation of bioethanol from fruit waste. Solid loading rate and reaction time for SSF had significant effect on production of bioethanol. Optimized 41.19 gL^-1 bioethanol concentration was observed with solid load rate of 20% (w/w) and fermentation period of 58.8 h. High yield of bioethanol can be achieved using fruit waste at domestic scale with minimum operational requirements.

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References

Ahmed, B., Mabrouk, K., Cherif, K. & Boudjemaa, B. (2016). Bioethanol production from date palm fruit waste fermentation using solar energy. African J. Biotechnol., 15: 1621–1627 DOI: https://doi.org/10.5897/AJB2016.15368

Akia, M., Yazdani, F., Motaee, E., Han, D. & Arandiyan, H. (2014). A review on conversion of biomass to biofuel by nanocatalysts. Biofuel Research Journal, 1(1):16-25. DOI: https://doi.org/10.18331/BRJ2015.1.1.5

Alaswad, A., Dassisti, M., Prescott, T. & Olabi, A.G. (2015). Technologies and developments of third generation biofuel production. Renewable and Sustainable Energy Reviews, 51:1446-1460 DOI: https://doi.org/10.1016/j.rser.2015.07.058

Ali, M.M., Anwar, R., Shafique, M.W., Yousef, A.F. & Chen, F. (2021a). Exogenous Application of Mg, Zn and B Influences Phyto-Nutritional Composition of Leaves and Fruits of Loquat (Eriobotrya japonica Lindl.). Agronomy, 11(2): 224. DOI: https://doi.org/10.3390/agronomy11020224

Ali, M.M., Anwar, R., Yousef, A.F., Li, B., Luvisi, A., Bellis, L. De, Aprile, A. & Chen, F. (2021b). Influence of Bagging on the Development and Quality of Fruits. Plants, 10(2): 358. DOI: https://doi.org/10.3390/plants10020358

Bhatti, Z.A., Rajput, M.-H. & Maitlo, G. (2019). Impact of Storage Time, Rain and Quality of Molasses in the Production of Bioethanol. Mehran Univ. Res. J. Eng. Technol., 38: 1021–1032. DOI: https://doi.org/10.22581/muet1982.1904.14

Bouhlali, E. dine T., Alem, C., Ennassir, J., Benlyas, M., Mbark, A.N. & Zegzouti, Y.F. (2017). Phytochemical compositions and antioxidant capacity of three date (Phoenix dactylifera L.) seeds varieties grown in the South East Morocco. J. Saudi Soc. Agric. Sci., 16: 350–357. DOI: https://doi.org/10.1016/j.jssas.2015.11.002

Carli, R., Dotoli, M. & Pellegrino, R. (2018). A decision-making tool for energy efficiency optimization of street lighting. Computers & Operations Research, 96:223-235. DOI: https://doi.org/10.1016/j.cor.2017.11.016

Colombini, K. (2020). RFA Urges Immediate EPA Action On ‘Litany’ Of Outstanding RFS Issues. Renewable Fuels Association. https://ethanolrfa.org/2020/08/rfa-urges-immediate-epa-action-on-litany-of-outstanding-rfs-issues/ [Accessed 22 September 2020].

El-Sohaimy, S.A. & Hafez, E.E. (2010). Biochemical and nutritional characterizations of date palm fruits (Phoenix dactylifera L.). J. Appl. Sci. Res., 6: 1060–1067.

Estefan, G., Sommer, R. & Ryan, J. (2013). Methods of Soil , Plant , and Water Analysis : A manual for the West Asia and North. Int. Cent. Agric. Res. Dry Areas.

Fakruddin, M. (2013). Process Optimization of Bioethanol Production by Stress Tolerant Yeasts Isolated From Agro-Industrial Waste. Int. J. Renew. Sustain. Energy, 2: 133. DOI: https://doi.org/10.11648/j.ijrse.20130204.11

Hashim, B.M., Sultan, M.A., Al Maliki, A. & Al-Ansari, N. (2020). Estimation of Greenhouse Gases Emitted from Energy Industry (Oil Refining and Electricity Generation) in Iraq Using IPCC Methodology. Atmosphere, 11(6):662. DOI: https://doi.org/10.3390/atmos11060662

Hughes, S.R. & Jones, M.A. (2020). Fuel Ethanol Production from Lignocellulosic Materials Using Recombinant Yeasts. Green Energy to Sustainability: Strategies for Global Industries, 269-282.. DOI: https://doi.org/10.1002/9781119152057.ch12

IEA. 2020. Global Annual Average Change in Energy Production By Fuel, 1971-2018 –Charts – Data & Statistics - IEA. https://www.iea.org/data-and-statistics/charts/global-annual-average-change-in-energy-production-by-fuel-1971-2018 [Accessed 9 September 2020].

Jursová, S., Burchart-Korol, D., Pustějovská, P., Korol, J. & Blaut, A. (2018). Greenhouse gas emission assessment from electricity production in the Czech Republic. Environments, 5(1):17 DOI: https://doi.org/10.3390/environments5010017

Kamzon, M.A., Abderafi, S. & Bounahmidi, T. (2016). Promising bioethanol processes for developing a biorefinery in the Moroccan sugar industry. International Journal of Hydrogen Energy, 41(45):20880-20896. DOI: https://doi.org/10.1016/j.ijhydene.2016.07.035

Makur, M.M. & Birhanu, T. (2020). Review on the Production and Characterization of Glucose and Ethanol from Sugarcane Bagasse. International Research Journal of Science and Technology, 1(2):169-176 DOI: https://doi.org/10.46378/irjst.2020.010214

Poorter, H. (2004). Physiological plant ecology. 4th edn. Ann. Bot. 93: 616–617. DOI: https://doi.org/10.1093/aob/mch084

Singh, D., Sharma, D., Soni, S.L., Sharma, S., Sharma, P.K. & Jhalani, A. (2020). A review on feedstocks, production processes, and yield for different generations of biodiesel. Fuel, 262:116553. DOI: https://doi.org/10.1016/j.fuel.2019.116553

Srivastava, N., Rawat, R., Sharma, R., Oberoi, H.S., Srivastava, M. & Singh, J. (2014). Effect of nickel–cobaltite nanoparticles on production and thermostability of cellulases from newly isolated thermotolerant Aspergillus fumigatus NS (Class: Eurotiomycetes). Applied biochemistry and biotechnology, 174(3):1092-1103. DOI: https://doi.org/10.1007/s12010-014-0940-0

Umar, T., Egbu, C. & Ofori, G. (2020). Challenges towards Renewable Energy: An Exploratory Study from GCC Region. Energy, 173(2): 1-34. DOI: https://doi.org/10.1680/jener.19.00034

WEC. 2020. World Energy Issues Monitor | 2017: Exposing The New Energy Realities. https://www.worldenergy.org/publications/entry/world-energy-issues-monitor-2017-exposing-the-new-energy-realities [Accessed 30 September 2020].

WHO. 2020. Fuel For Life: Household Energy And Health. https://www.who.int/indoorair/publications/fuelforlife/en/ [Accessed 15 September 2020].

Zabed, H., Faruq, G., Sahu, J.N., Azirun, M.S., Hashim, R. & Nasrulhaq Boyce, A. (2014). Bioethanol production from fermentable sugar juice. The Scientific World Journal, 2014: 1-11. DOI: https://doi.org/10.1155/2014/957102

Zabed, H., Sahu, J.N., Suely, A., Boyce, A.N. & Faruq, G. (2017). Bioethanol production from renewable sources: Current perspectives and technological progress. Renew. Sustain. Energy Rev., 71: 475–501.. DOI: https://doi.org/10.1016/j.rser.2016.12.076