Mutagen-induced Genetic Variability and Heritability Analysis for Yield Associated Quantitative Characters in Canola

Zeenat Bano; Sadaf Tabasum Qureshi; Rabia Asma Memon; Imtiaz Ahmed

doi:10.38211/joarps.2025.06.300

Authors

Zeenat Bano Institute of Plant Sciences, University of Sindh, Jamshoro, Pakistan
Sadaf Tabasum Qureshi Institute of Plant Sciences, University of Sindh, Jamshoro, Pakistan
Rabia Asma Memon Institute of Plant Sciences, University of Sindh, Jamshoro, Pakistan
Imtiaz Ahmed Nuclear Institute of Agriculture, Tando Jam

DOI:

https://doi.org/10.38211/joarps.2025.06.300

Keywords:

Mutagen, yield, quantitative characters, Canola, Germ plasm

Abstract

Due to adverse effect of other cooking oils, people are switched on health promoting oils and vegetables; therefore canola genetic improvement regarding yield has been planned in majority of European and Asian countries. The major advantage of canola green and oil is for availability unsaturated fatty acid which indirectly improves blood pressure by thinning blood (dissolve many nutrients, vitamins and cholesterol) cardiac activities and muscles strength. Genetic variability through mutagenesis is proven as a powerful tool for introducing new alleles, therefore different doses of Gamma radiations (750Gy, 1000Gy, 1250Gy) and Ethyl methansalfonate (0.5%, 1.0% and 1.5%) were used for grain yield associated quantitative characters in two Canola genotypes (Hyola-42 and Shiralee). The data was recorded for number of seeds per pod, number of pods per plant, 1000 seed weight and seed yield per plant in M2 and M3 generation. All mutagenic treatments had a significant (p≤0.05) variation on all traits studied and produced great variability for both mutagens. The mean value of all the phenotypic parameters in M2 generation increased with moderate dose of EMS and Gamma rays in both genotypes as compared to respective controls and in M3 generation both genotypes were more responsive to Gamma rays (1000Gy) for all studied characters in both tested genotypes. Heritability analysis exhibited high heritability and high genetic advance for most of the traits by moderate and highest does of Gamma rays, while reverse is true for EMS in both generations for Shiralee. Over all gamma rays was most efficient to induce better allele contributing to 1000-seed weight.

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References

Soybeans (Glycine Max (L.) Merill) irradiated with Cobalt–60 Gamma Irradiation in the Guinea Savannah Agroecology of Ghana. Ghana Journal of Science, Technology and Development, 6(1), 30-37.

Ali, H.M.A., and Shah, S. A. (2013). Evaluation and selection of rapeseed (Brassica napus L.) mutant lines for yield performance using augmented design. Journal of Animal and Plant Science. 23: 1125–1130.

Amosova, A.V., Zoshchuk, S.A., Volovik, V.T., Shirokova, A.V., Horuzhiy, N.E., and Mozgova, G.V. (2019). Phenotypic, biochemical and genomic variability in generations of the rapeseed (Brassica napus L.) mutant lines obtained via chemical mutagenesis. Public Library of Science.(PLOS ONE)14(8):e0221699.

Anwar, M. M., Ali, S. E., & Nasr, E. H. (2015). Improving the nutritional value of canola seed by gamma irradiation. Journal of Radiation Research and Applied Sciences, 8(3), 328-333.

Arulbalachandran, D., Mullainathan, L., Velu, S. and Thilagavathi, C. (2010). Genetic variability, heritability and genetic advance of quantitative traits in black gram by effects of mutation in field trail. African Journal of Biotechnology. 9: 2731-2735.

Bolbhat, S.N., Gawade, B.B., Bhoge, V.D., Wadavkar, D.S., Shendage, V.S., and Dhumal K.N. (2012). Effect of mutagens on frequency and spectrum of viable mutations in Horsegram (Macrotyloma uniflorum (Lam.) Verdc). Journal of Agriculture and Veterinary Science (IOSR-JAVS) ISSN: 2319-2380. 1,1(2012), PP45-55. www.iosrjournals.org. https://www.researchgate.net/publication/272712049.

Braatz, J., Harloff, H.J., Mascher, M., Stein, N., Himmelbach, A., and Jung, C. (2017). CRISPR-Cas9 targeted mutagenesis leads to simultaneous modification of different homologous gene copies in polyploidy oilseed rape (Brassica napus). Plant Physiology. 174 (2): 935–942.

Bus, A., Korber, N., Snowdon, R.J., and Stich, B. (2011). Patterns of molecular variation in a species-wide germplasm set of Brassica napus. Theoretical and Applied Genetics, 123(8): 1413–1423.

Chaudhary, J., Deshmukh, R., and Sonah, H. (2019). Mutagenesis approaches and their role in crop improvement. Plants, 8 (11), 467

Emrani, S.M., Arzani, A., Saidi, G., Abtahi, M., Banifatimeh, M., Parsa, M.B. and Fatokian, M.H. (2012). Evaluation of induced genetic variability in agronomic traits by Gamma irradiation in canola (Brassica napus L.). Pakistan Journal of Botany. 44(4): 1281 – 1288.

Emrani, S.N., Harloff, H., Gudi, O., Kopisch, Obuch, F., and Jung, C. (2015). Reduction in sinapine content in rapeseed (Brassica napus L.) by induced mutations in Sinapine biosynthesis genes. Molecular Breeding, 35 (1): 37.

Falconer, D.S. (1977). Introduction to quantitative genetics. Richard Clay Ltd. Bungay Suffolk, Great Britain Pp.129-185.

Gupta, R., Mcroberts, R., Yu, Z., Smith, C., Saloan, W., and You, S. (2022). life cycle assessment of biodiesel production from rapeseed oil, inflorescence of process parameters and scale. Bioresources Technology, 360-127532s.

Gupta, S., Kumar, A., Janeja, H.S., Prakash, A., and Anand, R. (2024). Genetic engineering in Indian Mustard (Brassica juncea L.): current progress and future directions for enhanced crop improvement. Journal of Advances in Biology & Biotechnology. 27(5):739-751

Hasan, M., Seyis, F., and Badani, A.G. (2006). Analysis of genetic diversity in the Brassica napus L. gene pool using SSR markers. Genetics and Resources of Crop Evolution, 53(4): 793–802.

Hussain, S., Khan, W.M. and Khan, M. S. (2017). Mutagenic effect of sodium azide (NaN3) on M2 generation of Brassica napus L. (variety Dunkled). Pure Applied Biology, 6: 226–236.

Hussein, O.S. (2012). Protein electrophoresis and DNA in herbs produced from irradiated Amrosia maritime seeds grown under soil salinity and their resistance to insects. American Journal of Pant.Physiology. 7(6): 261-268.

Javeed, M.A., Khatri, A., Khan, I.A., Ahmed, M., Siddiqui, M.A., and Arain, A.G. (2000). Utilization of gamma irradiation for the genetic improvement of oriental mustard (Brassia Juncea Coss). Pakistan Journal of Biological Sciences, 32(1)77-83.

Khan, W.M., Shah, S.Z., Khan, M.S., Islam, Z.U., Ali, S., Hussain, F., Irshad, M., and Zahid, M. (2014). Effects of gamma radiations on some morphological and biochemical characteristics of Brassica napus L. (variety Altex). International Journal of Biological Sciences. 4(10):36-41, .

Larik, A.S., and Rajput, L.S. (2000). Estimation of selection indices in Brassica junceae L., Brassica napus L,. Pakistan Journal of Biological Sciences, 32: 323-330.

Lee, Y.H., Park, W., and Kim, K.S. (2018). EMS-induced mutation of an endoplasmic reticulum oleate desaturase gene (FAD2-2) results in elevated oleic acid content in rapeseed (Brassica napus L.). Euphytica 214: 28.

Okuzaki, A., Ogawa, T., and Koizuka, C. (2018). CRISPR/Cas9-mediated genome editing of the fatty acid desaturase 2 gene in Brassica napus. Plant Physiology and Biochemistry, 131: 63–69.

Panda, S., Biswas, C. K., & Paul, S. (2024). Production of Biodiesel from Industrial Sludge: Recent Progress, Challenges, Perspective. Recent Trends in Management and Utilization of Industrial Sludge, 337-357.

Parry, M.A., Madgwick, P.J., and Bayon, C. (2009). Mutation discovery for crop improvement. Journal of Experimental Botany 60: 2817–2825.

Singh, R.K., and Chandhri, B.D. (1985). Biometrical methods in quantitative genetics analysis. Kalalyani Pub. Ludhiana. India, Pp. 9-39.

Shah, S.A., Ali, I., and Rahman, K. (1990). Induction and selection of superior genetic variables of oilseed rape, Brassica napus L. The Nucleus, 7: 37-40.

Sharafi, Y., Majidi, M.M., Goli, S.H., and Rashidi, F. (2015). Oil content and fattyacids composition in Brassica species. International Journal of Food Propagation. 18: 2145–2154.

Siddiqui, M.S., Khan, I.A., and Khatri, A. (2009). Induced quantitative variability by Gamma rays and Ethylmethane sulphonate alone and in combination in rapeseed (Brassica napus L.). Pakistan Journal of Biological Sciences, 41(3): 1189-1195.

Shah, M.A., Rehman, F.U., Mehmood, A., Ullah, F., Shah, S.I., and Rasheed, S.M. (2021).Combining ability, heritability and gene action assessment in rapeseed (Brassica napus L.) for yield and yield attributes. Sarhad Journal of Agriculture, 37(1): 104-109.

Siddiqui, M.A., Aslam, M,M., Sial, M.A., Soomro, N.S., Khan, M.T., Yasmeen, S., Sial, S., and Khan, I.A. (2021). Surhan-2012: A novel high yielding, insect resistant, and high oil content canola variety released for general cultivation in the Sindh Province. Pakistan Journal of Agricultural Research, 34(4):837-845.

STATIST (2021).

Uzair, M., Shahzadi, I., Jatoi, G.H., Bibi, T., Rauf, S., Mahmood, T., and Salah-ud-Din. (2016). Genetic variability and heritability studies in relation to seed yield and it’s components traits in Mustard (Brassica juncea L.). Academia Journal of Agricultural Research, 4(8): 478-482, DOI: 10.15413/ajar.2016.0177

Zeng, Q., and Liu, K. (2022). Worldwide rapeseed (Brassica napus L.) research: A bibliometric analysis during 2011–2021. Oil Crop Science. 7, (4), Pp157-165.